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eclipse ti-e widefield microscope  (Nikon)

 
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    Nikon eclipse ti-e widefield microscope
    a. Relative brightness (average ± sd) of mScarlet3 and mScarlet3-H in HeLa cells 24 h after transfection, determined by normalized, spectrally corrected ratios of red to cyanfluorescence intensity found in cells upon co-production of RFP and mTurquoise2. b. maturation speed of mScarlet3-H in HeLa cells measured as delay (average ± sd in min) relative to co-produced mTurquoise2, n=8 cells. c. Photobleaching kinetics of mScarlet and mScarlet3 in living HeLa cells. d. Photobleaching kinetics of mScarlet-H and mScarlet3-H in living HeLa cells. For c. and d. cells were subjected to <t>widefield</t> microscopy and illuminated at ∼ 4W/cm2 with 550/15 nm LED power. Only backgroundfluorescence was subtracted and the netfluorescence intensity was normalized to the initial value at t=0. The average ± sd of four individual cells is shown for each curve.
    Eclipse Ti E Widefield Microscope, supplied by Nikon, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/eclipse ti-e widefield microscope/product/Nikon
    Average 90 stars, based on 1 article reviews
    eclipse ti-e widefield microscope - by Bioz Stars, 2026-03
    90/100 stars

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    1) Product Images from "mScarlet3-H with low brightness and fluorescence lifetime has potential for cellular lifetime-unmixing and lifetime-based pH-sensing applications"

    Article Title: mScarlet3-H with low brightness and fluorescence lifetime has potential for cellular lifetime-unmixing and lifetime-based pH-sensing applications

    Journal: bioRxiv

    doi: 10.1101/2025.07.15.664898

    a. Relative brightness (average ± sd) of mScarlet3 and mScarlet3-H in HeLa cells 24 h after transfection, determined by normalized, spectrally corrected ratios of red to cyanfluorescence intensity found in cells upon co-production of RFP and mTurquoise2. b. maturation speed of mScarlet3-H in HeLa cells measured as delay (average ± sd in min) relative to co-produced mTurquoise2, n=8 cells. c. Photobleaching kinetics of mScarlet and mScarlet3 in living HeLa cells. d. Photobleaching kinetics of mScarlet-H and mScarlet3-H in living HeLa cells. For c. and d. cells were subjected to widefield microscopy and illuminated at ∼ 4W/cm2 with 550/15 nm LED power. Only backgroundfluorescence was subtracted and the netfluorescence intensity was normalized to the initial value at t=0. The average ± sd of four individual cells is shown for each curve.
    Figure Legend Snippet: a. Relative brightness (average ± sd) of mScarlet3 and mScarlet3-H in HeLa cells 24 h after transfection, determined by normalized, spectrally corrected ratios of red to cyanfluorescence intensity found in cells upon co-production of RFP and mTurquoise2. b. maturation speed of mScarlet3-H in HeLa cells measured as delay (average ± sd in min) relative to co-produced mTurquoise2, n=8 cells. c. Photobleaching kinetics of mScarlet and mScarlet3 in living HeLa cells. d. Photobleaching kinetics of mScarlet-H and mScarlet3-H in living HeLa cells. For c. and d. cells were subjected to widefield microscopy and illuminated at ∼ 4W/cm2 with 550/15 nm LED power. Only backgroundfluorescence was subtracted and the netfluorescence intensity was normalized to the initial value at t=0. The average ± sd of four individual cells is shown for each curve.

    Techniques Used: Transfection, Produced, Microscopy



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    a. Relative brightness (average ± sd) of mScarlet3 and mScarlet3-H in HeLa cells 24 h after transfection, determined by normalized, spectrally corrected ratios of red to cyanfluorescence intensity found in cells upon co-production of RFP and mTurquoise2. b. maturation speed of mScarlet3-H in HeLa cells measured as delay (average ± sd in min) relative to co-produced mTurquoise2, n=8 cells. c. Photobleaching kinetics of mScarlet and mScarlet3 in living HeLa cells. d. Photobleaching kinetics of mScarlet-H and mScarlet3-H in living HeLa cells. For c. and d. cells were subjected to widefield microscopy and illuminated at ∼ 4W/cm2 with 550/15 nm LED power. Only backgroundfluorescence was subtracted and the netfluorescence intensity was normalized to the initial value at t=0. The average ± sd of four individual cells is shown for each curve.

    Journal: bioRxiv

    Article Title: mScarlet3-H with low brightness and fluorescence lifetime has potential for cellular lifetime-unmixing and lifetime-based pH-sensing applications

    doi: 10.1101/2025.07.15.664898

    Figure Lengend Snippet: a. Relative brightness (average ± sd) of mScarlet3 and mScarlet3-H in HeLa cells 24 h after transfection, determined by normalized, spectrally corrected ratios of red to cyanfluorescence intensity found in cells upon co-production of RFP and mTurquoise2. b. maturation speed of mScarlet3-H in HeLa cells measured as delay (average ± sd in min) relative to co-produced mTurquoise2, n=8 cells. c. Photobleaching kinetics of mScarlet and mScarlet3 in living HeLa cells. d. Photobleaching kinetics of mScarlet-H and mScarlet3-H in living HeLa cells. For c. and d. cells were subjected to widefield microscopy and illuminated at ∼ 4W/cm2 with 550/15 nm LED power. Only backgroundfluorescence was subtracted and the netfluorescence intensity was normalized to the initial value at t=0. The average ± sd of four individual cells is shown for each curve.

    Article Snippet: After 24 h, fluorescence images were collected using a Nikon Eclipse Ti-E widefield microscope with LEDs at 440 and 555 nm (SpectraX, Lumencor).

    Techniques: Transfection, Produced, Microscopy